Countess® II Automated Cell Counter自动化细胞计数仪描述
The new Countess™ II Automated Cell Counter is a benchtop assay platform equipped with state-of-the-art optics, full autofocus, and image analysis software for rapid assessment of cells in suspension.
• Accurate—use the Countess II Automated Cell Counter to eliminate the subjectivity of manual cell counting and user-to-user variability
• Fast—the Countess II Automated Cell Counter provides a total cell count, reports viability, and measures average cell size in as little as 10 seconds
• Convenient—the Countess II Automated Cell Counter requires no cleaning or routine maintenance. A capacitive touchscreen and simple user interface provides quick startup and requires minimal training.
The Countess II Automated Cell Counter comes with:
• Countess USB drive for storing and transferring data to your computer
• Disposable slide holder
• Power cord and 4 plug adaptors to accommodate most countries
• Quick reference card (QRC)
• A free box of 50 disposable slides
The Countess II Automated Cell Counter works in 3 simple steps:
1. Mix 10 µL of sample with 10 µL of trypan blue and pipet into a disposable Countess chamber slide.
2. Insert the slide into the instrument.
3. Press the "Count" button.
Extended Warranty Packages
Protect your instrument and your investment with the Countess II Automated Cell Counter Extended Warranty Packages. Save up to 25% off the individual instrument and extended warranty list prices when purchased together.
Get accurate cell counts
The Countess II Automated Cell Counter uses trypan blue staining combined with an auto-focus mechanism and sophisticated image analysis algorithm to obtain accurate cell and viability counts. The measurement range extends from 1 x 10e4 to 1 x 10e7 cells/mL (the optimal range is 1 x 10e5 to 4 x 10e6 cells/mL), which is broader than that of a hemocytometer. The optimal cell size for accurate viability assessment is 7–60 µm, while particles as small as 4 µm can be successfully counted.
Transfer data and images to your PC
Using the USB port on the Countess II Automated Cell Counter, you can save your cell count results and images, and then transfer them to your PC. (For your convenience, a USB drive is supplied with the Countess II instrument).
For Research Use Only. Not for use in diagnostic procedures.
产品仅供科研使用，不用于临床诊断。
Countess全自动细胞计数仪选型指南 只需几秒即可对细胞精确计数
Thermo Fisher Scientific提供了两款可符合任意实验室应用要求的自动化细胞计数仪。您可以选择简单地明场计数的Invitrogen™ Countess™ II自动化细胞计数仪，或者选择可以灵活升级的荧光检测计数Invitrogen™ Countess™ II FL自动化细胞计数&荧光分析仪。
这两款细胞计数仪均可提供以下优势： 精确 —消除了手动细胞计数的主观性、最大限度减少了人为误差 快速—可以同时计算活/死细胞，测定细胞活力和细胞平均大小，耗时不过10秒 方便—配有一面电容式触摸屏和简单的用户界面，可以快速上手，最大限度缩减了培训工作量 Countess II和Countess II FL自动化细胞仪之比较
先进的自动聚焦和计数算法
Countess自动化细胞计数仪含有先进的自动聚焦和计数算法，可以快速、精确地识别并计数细胞群之中的细胞（图1）。Countess 仪器兼容的细胞类型和细胞系广泛， 我们已经采用众多通用细胞系对其进行了验证。将玻片插入到Countess仪器之中以后，系统即会对细胞进行自动对焦，这将最大限度减少与手动对焦相关的 人为波动。这两款细胞仪最适于计数大小介于~7 µm和60 µm之间，悬液密度为1 x 104 - 1 x 107 细胞/mL的细胞。 通用细胞系验证表Cell line validation
Counting has been successfully performed on the following cell lines using the Countess II FL Automated Cell Counter:
Cell
Animal
Organ
A431
Human
Skin
Adipocytes
Human
Adipose-derived stem cells
Aortic Smooth Muscle
Human
Smooth muscle
CHO-M1WT2
Chinese hamster
Ovary
CHSE
Chinook salmon
Embryo
COLO-205
Human
Colon
COS-7
African monkey
Kidney
HeLa
Human
Cervix
HepG2
Human
Liver
HL-60
Human
Blood
J774A.1
Mouse
Blood
Jurkat
Human
Blood
K562
Human
Bone marrow
MCF7
Human
Breast
MRC-5
Human
Lung
NIH/3T3
Mouse
Embryo
PBMC
Human
Blood
PC-12
Rat
Adrenal gland
Pulmonary artery endothelial
Human
Blood vessel
Pulmonary artery smooth muscle
Human
Smooth muscle
SF-21
Insect
Ovary
U266
Human
Blood
U2OS
Human
Bone
Umbilical vein endothelial
Human
Blood vessel
Whole lysed blood
Human
Blood
图1. 自动聚焦和先进的计数算法可以实现更精确的计数。
采用Countess II FL全自动细胞计数仪（蓝条）或显微镜和细胞计手动计数（紫条）计数的NIH/3T3、HeLa和HL-60细胞。每一个竖条代表10次计数的平均值。
