升级后的Mini-Sub cell GT 电泳槽使电泳分离更加容易。 Mini-Sub cell GT 电泳槽可分离多达 30 个样品;短而窄的电泳槽支持7 和 10 cm 凝胶快速、简便、经济地实验运行。
Bio-Rad 小型电泳槽在 60 V 电压下分离 lambda DNA 的 EcoRI 或 HindIII 酶切产物,只需 1.5 小时。在 150 V 下,小型 DNA 片段在短短 15 分钟内即可分离。
所有小型电泳槽都兼容 ReadyAgarose 预制胶,可以节省时间并允许进行高度可重复的分离,这些小型电泳槽包括一个缓冲液槽、一个带电缆的安全盖和一个水平测量器。 同时,电泳系统还提供了包括其他可选配件(有关更多详细信息,请参见“Mini-Sub Cell GT 完整系统选择指南”)。
功能:
- QuickSnap* 电极拆卸方便,简化清洁工作
- 底座侧面的箭头指示运行方向,可确保正确的凝胶方向
- 彩色编码且带标记的电极和带标记的底座确保盖子可以正确安装在底座上
- 基部拉环设计,可轻松移除盖子,从而减少缓冲液溢出量,还可防止盖位置不正确
- 反向兼容设计确保这些电泳槽兼容较早型号的配件
- 透明塑料结构,确保实时便捷地查看实验进程
- 具有荧光标尺的紫外透明凝胶托盘**
- 铺制门可方便的在凝胶槽内直接铺制,或者用于无胶条式灌制的可选制胶盘**
- 可满足一切需求的电泳梳(高度固定的嵌入式梳、可调节高度的电泳疏和制备电泳梳)**
** 请单独购买 ReadySub-Cell GT 系统。
Mini ReadySub-Cell GT 电泳槽
小型 ReadySub-Cell GT 电泳槽与 Mini-Sub cell GT 电泳槽相同,但小型ReadySub-Cell GT 电泳槽专用于跑 ReadyAgarose 预制胶。 预制胶可牢固固定到小型 ReadySub-Cell 室内,操作方便且具有可重复性。 此电泳槽不包括铺制门、凝胶盘或电泳梳。 试剂盒可用于升级至 ReadySub-Cell GT cell,以支持手灌功能。
Mini-Sub® Cell GT 完整系统*
货号 |
铺制门 |
制胶盘 |
UVTP 托盘 (cm) |
梳 |
PowerPac Basic
电源 |
7 x 7** |
7 x 10 |
8 孔 |
15 孔 |
170-4406 |
• |
|
• |
|
• |
• |
|
170-4466 |
|
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|
• |
• |
• |
|
170-4486 |
• |
• |
• |
|
• |
• |
|
170-4467 |
|
• |
|
• |
• |
• |
|
164-0300 |
|
• |
|
• |
• |
• |
• |
170-4487 |
|
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|
|
|
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|
164-0303 |
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|
|
|
|
|
• |
* 1996 年以前购买的 Mini-Sub 电泳槽系统(Mini-Sub DNA 电泳槽)需要凝胶盘(货号 170-4330)。 该托盘与 Mini-Sub cell GT 系统不兼容。
Mini-Sub® Cell 电泳梳选择指南
货号 |
孔数量 |
高度* |
厚度 |
宽度孔 |
齿梳长度 |
体积
(在 5 mm 深凝胶中) |
Mini-Sub Cell GT 系统的梳 |
170-4464 |
15 |
固定 |
0.75 mm |
3 mm |
10 mm |
11.25 µl |
170-4465** |
15 |
固定 |
1.5 mm |
3 mm |
10 mm |
22.5 µl |
170-4332 |
15 |
可调 |
1 mm |
2.6 mm |
10.2 mm |
13 µl |
170-4462 |
8 |
固定 |
0.75 mm |
5.5 mm |
11 mm |
20 µl |
170-4463** |
8 |
固定 |
1.5 mm |
5.5 mm |
11 mm |
40 µl |
170-4333 |
8 |
可调 |
1 mm |
5.5 mm |
10.2 mm |
27.5 µl |
170-4461 |
2 制备 |
固定 |
1.5 mm |
20 mm |
10 mm |
150 µl |
|
2 参考 |
|
|
4 mm |
|
30 µl |
170-4460 |
1 制备 |
固定 |
1.5 mm |
44 mm |
10 mm |
330 µl |
|
2 参考 |
|
|
3 mm |
|
22.5 µl |
170-4342 |
1 制备 |
可调 |
3 mm |
43.3 mm |
10.2 mm |
645 µl |
|
2 参考 |
|
|
3 mm |
|
45 µl |
* 固定高度电泳梳必须与 GT 凝胶托盘一起使用。 可调节高度的电泳梳需具备电泳梳支架,目录编号为 170-4331。
** 系统中附含电泳梳 .
技术参数:
电泳槽尺寸(宽 x 长 x 高) |
9.2 x 25.5 x 5.6 厘米 |
凝胶托盘尺寸 (OD)(宽 x 长) |
7 x 7 厘米
7 x 10 厘米 |
是否兼容 ReadyAgarose™ 凝胶 |
是,8 孔、12 孔、2 x 8 孔 |
样本通量 |
8–30 |
基本缓冲液需要量 |
~270 ml |
缓冲液再循环 |
否 |
溴酚蓝迁移 |
~4.5 cm/hr(75 V 下) |
订购信息:
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Mini-Sub Cell GT System 1704406
Mini horizontal electrophoresis system, includes 8- and 15-well combs, 7 x 7 cm UV-transparent tray, casting gates |
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Mini-Sub® Cell GT System 1704466
Mini horizontal electrophoresis system, includes 8- and 15-well combs, 7 x 10 cm UV-transparent tray |
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Mini-Sub® Cell GT System 1704467
Mini horizontal electrophoresis system, includes 8- and 15-well combs, 7 x 10 cm UV-transparent tray, mini-gel caster |
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Mini-Sub Cell GT Cell and PowerPac Basic Power Supply 1640300
Mini horizontal electrophoresis system, includes 8- and 15-well combs, gel caster, 7 x 10 cm UV-transparent tray, basic power supply (#164-5050) |
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Mini ReadySub-Cell™ GT Cell 1704487
Horizontal electrophoresis cell, includes lid and electrodes, accommodates 8- and 12-well mini ReadyAgarose™ gels |
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Mini ReadySub-Cell™ GT Cell and PowerPac™ Basic Power Supply 1640303
Horizontal electrophoresis cell system, accommodates 8- and 12-well mini ReadyAgarose™ gels, includes basic power supply (#164-5050) |
配件:
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Mini Handcasting Kit 1704491
Agarose mini-gel casting kit, includes 7 x 7 UV-transparent tray, 15 x 7 casting gates, 8- and 15-well 1.5 mm fixed height combs |
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Mini-Gel Caster 1704422
Pkg of 1, gel caster for use with Mini-Sub and wide Mini-Sub cell GT systems |
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Sub-Cell GT UV-Transparent Mini-Gel Tray 1704436
Pkg of 1, 7 x 7 cm gel tray, allows casting gels in cells using casting gates, 2 slots for fixed-height combs, for use with Mini-Sub cell GT systems |
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Sub-Cell GT UV-Transparent Mini-Gel Tray 1704435
Pkg of 1, 7 x 10 cm gel tray, 2 slots for fixed-height combs, for use with Mini-Sub cell GT systems |
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Original UV-Transparent Mini-Gel Tray 1704330
Pkg of 1, 7 x 10 cm, horizontal agarose gel electrophoresis casting tray, for use with Mini-Sub cell systems purchased before 1996 (Mini-Sub DNA cell) |
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Mini-Sub Cell GT Casting Gates 1704434
Pkg of 2, horizontal agarose gel electrophoresis casting gates, for use with Mini-Sub cell GT systems |
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Mini-Sub Cell GT Anode (Red) Electrode Assembly 1704432
Pkg of 1, electrode assembly for use with Mini-Sub cell GT systems, older models |
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Mini-Comb Holder 1704331
Pkg of 1, horizontal electrophoresis comb holder, for use with Mini-Sub cell GT adjustable-height combs |
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Mini-Sub Cell GT Anode (Red), QuickSnap Electrode Assembly 1704362
Pkg of 1, electrode assembly for use with Mini-Sub cell GT systems, 2009 model |
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Mini-Sub Cell GT Cathode (Black), QuickSnap Electrode Assembly 1704363
Pkg of 1, electrode assembly for use with Mini-Sub cell GT systems, 2009 model |
梳子:
|
15-Well Comb 1704464
Pkg of 1, 0.75 mm thick, fixed-height comb, for use with Mini-Sub cell GT systems, must use with GT gel tray |
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15-Well Comb 1704465
Pkg of 1, 1.5 mm thick, fixed-height comb, for use with Mini-Sub cell GT systems, must use with GT gel tray |
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15-Well Comb 1704332
Pkg of 1, 1.0 mm thick, adjustable-height comb, for use with Mini-Sub cell GT systems, requires comb holder (#170-4331) |
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8-Well Comb 1704462
Pkg of 1, 0.75 mm thick, fixed-height comb, for use with Mini-Sub cell GT systems, must use with GT gel tray |
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8-Well Comb 1704463
Pkg of 1, 1.5 mm thick, fixed-height comb, for use with Mini-Sub cell GT systems, must use with GT gel tray |
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8-Well Comb 1704333
Pkg of 1, 1.0 mm thick, adjustable-height comb, for use with Mini-Sub cell GT systems, requires comb holder (#170-4331) |
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Preparative Comb 1704461
Pkg of 1, 1.5 mm thick, includes 2 preparative and 2 reference wells, fixed height, for use with Mini-Sub cell GT systems, must use with GT gel tray |
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Preparative Comb 1704460
Pkg of 1, 1.5 mm thick, includes 1 preparative and 2 reference wells, fixed height, for use with Mini-Sub cell GT systems, must use with GT gel tray |
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Preparative Comb 1704342
Pkg of 1, 3 mm thick, preparative comb with 1 preparative and 2 reference wells, adjustable height, requires comb holder (#170-4331) |
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