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美国BIO-RAD伯乐 Trans-Blot® Turbo™ 蛋白转印系统 |
Trans-Blot® Turbo™ Transfer Starter System: 1704155 (含启动耗材)
Blotting instrument, includes base, 2 cassettes to hold 1–2 midi or up to 4 mini blotting sandwiches, blot roller, and starter consumable kit .
Trans-Blot® Turbo™ Transfer System: 1704150
Blotting instrument, includes base, 2 cassettes to hold 1-2 midi or up to 4 mini blotting sandwiches, blot roller |
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Trans-Blot Turbo转印系统结合了传统转印技术与现代印迹膜及滤纸合,可在最短的时间内完成蛋白质从凝胶到膜的转印,且操作非常简便。系统内置了高电流电源, 可直接控制钛阳极和不锈钢阴极间的电流,能在3分钟内实现快速转印。全套系统采取即用型设计,研究人员可更快、更轻松的获得结果,并且具有传统印迹方 法难以实现的高重复性。
- 快速转印 — 只需 3 分钟即可转印小型凝胶或中型凝胶
- 高通量 — 单次运行可转印 1–4 块小型胶或 1–2 块中型胶
- 更高的转印效率 — 比其他转印方法的转印效率更高
- 灵活设计 — 允许用户自定义转移条件并与传统的半干式消耗品相容
- 环境友好 — 环保型耗才可节省废弃物处理费用
超高的转印效率
与其他印迹技术相比较,使用 Trans-Blot® Turbo™ 系统可获得更高的灵敏度和更好的转印效率。 这一数据集显示仅使用 Trans-Blot Turbo 系统成功转印 1.25 ng 蛋白条带。 在 Criterion™ TGX™ 4–20% 凝胶上分离转铁蛋白的系列稀释并使用四种不同印迹技术进行转移。 所有图像均调整至相同设置。
在 3、7 或 10 分钟内转移
使用 Trans-Blot® Turbo™ 转印包进行快速转印
3 分钟方案 — 在短短 3 分钟内完成 Mini-PROTEAN® TGX™ 凝胶的超快速转印(蛋白分子量范围:5–150 kD)
7 分钟方案 — 在 7 分钟内转印 4块个小型或 2 块中型凝胶(蛋白质分子量范围:5–150 kD)
10 分钟方案 — 在 10 分钟内转印 4块小型或 2 块中型凝胶(蛋白质分子量范围:25–300+ kD)
大肠杆菌 裂解物 (6 µg) 稀释了两倍。 样本用 Mini-PROTEAN TGX 凝胶进行分离,用 Trans-Blot Turbo 系统进行转移,用 SYPRO Ruby 进行染色,然后在 VersaDoc™ MP 4000 系统上进行成像。 通道 1 中的标准品是 Precision Plus Protein™ 未染标准品,顶部带为 250 kD MW。
更好的转印不同类型的蛋白
2x 蛋白质转移对使用 Trans-Blot® Turbo™ 和 iBlot 系统转印的等量 2-D 凝胶进行的定量表明使用 Trans-Blot Turbo 系统转印和检测到的蛋白数增加了一倍。 在 Bio-Rad 的 ReadyStrip™ 11 cm pH 5–8 的 IPG 胶条上聚焦大鼠肝提取物,并将其分离到 AnykD™ Criterion™ TGX™ 凝胶上。 使用 Trans-Blot Turbo 和 iBlot 系统转移复制凝胶,这两种系统都使用制造商 ’ 推荐的 7 分钟实验方案。 随后用 SYPRO Ruby 对硝化纤维素膜进行染色,并在 VersaDoc™ MP 4000 系统上进行成像。
Trans-Blot® Turbo™ 蛋白转印系统订购信息:
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Trans-Blot® Turbo™ Transfer Starter System
1704155
Blotting instrument, includes base, 2 cassettes to hold 1–2 midi or up to 4 mini blotting sandwiches, blot roller, and starter consumable kit |
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Trans-Blot® Turbo™ Transfer System
1704150
Blotting instrument, includes base, 2 cassettes to hold 1-2 midi or up to 4 mini blotting sandwiches, blot roller |
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Trans-Blot® Turbo™ Mini PVDF Transfer Packs
1704156
Pkg of 10, 7 x 8.5 cm, precut blotting transfer pack, includes filter paper, buffer, PVDF membrane, for use with Trans-Blot Turbo transfer system |
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Trans-Blot® Turbo™ RTA Mini PVDF Transfer Kit, for 40 blots
1704272
Ready-to-assemble transfer kit includes 40 mini-sized PVDF membranes (7 x 8.5 cm), 80 transfer stacks, 1 L 5x transfer buffer, and 2 gel trays for wetting and equilibrating membranes and transfer stacks |
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Trans-Blot® Turbo™ Midi PVDF Transfer Packs
1704157
Pkg of 10, 8.5 x 13.5 cm, precut blotting transfer pack, includes filter paper, buffer, PVDF membrane, for use with Trans-Blot Turbo transfer system |
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Trans-Blot® Turbo™ RTA Midi PVDF Transfer Kit, for 40 blots
1704273
Ready-to-assemble transfer kit includes 40 midi-sized PVDF membranes (8.5 x 13.5 cm), 80 transfer stacks, 2 L 5x transfer buffer, and 2 gel trays for wetting and equilibrating membranes and transfer stacks |
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Trans-Blot® Turbo™ Mini Nitrocellulose Transfer Packs
1704158
Pkg of 10, 7 x 8.5 cm, precut blotting transfer pack, includes filter paper, buffer, nitrocellulose membrane, for use with Trans-Blot Turbo transfer system |
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Trans-Blot® Turbo™ RTA Mini LF PVDF Transfer Kit, for 40 blots
1704274
Ready-to-assemble transfer kit includes 40 mini-sized LF PVDF membranes (7 x 8.5 cm), 80 transfer stacks, 1 L 5x transfer buffer, and 2 gel trays for wetting and equilibrating membranes and transfer stacks |
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Trans-Blot® Turbo™ Midi Nitrocellulose Transfer Packs
1704159
Pkg of 10, 8.5 x 13.5 cm, precut blotting transfer pack, includes filter paper, buffer, nitrocellulose membrane, for use with Trans-Blot Turbo transfer system |
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Trans-Blot® Turbo™ RTA Midi LF PVDF Transfer Kit, for 40 blots
1704275
Ready-to-assemble transfer kit includes 40 midi-sized LF PVDF membranes (8.5 x 13.5 cm), 80 transfer stacks, 2 L 5x transfer buffer, and 2 gel trays for wetting and equilibrating membranes and transfer stacks |
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Trans-Blot® Turbo™ RTA Mini Nitrocellulose Transfer Kit, for 40 blots
1704270
Ready-to-assemble transfer kit includes 40 mini-sized nitrocellulose membranes (7 x 8.5 cm), 80 transfer stacks, 1 L 5x transfer buffer, and 2 gel trays for wetting and equilibrating membranes and transfer stacks |
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Trans-Blot® Turbo™ Cassette
1704151
Pkg of 1, cassette, to hold 1 midi or up to 2 mini blotting sandwiches, for use with Trans-Blot Turbo transfer system |
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Trans-Blot® Turbo™ RTA Midi Nitrocellulose Transfer Kit, for 40 blots
1704271
Ready-to-assemble transfer kit includes 40 midi-sized nitrocellulose membranes (8.5 x 13.5 cm), 80 transfer stacks, 2 L 5x transfer buffer, and 2 gel trays for wetting and equilibrating membranes and transfer stacks |
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Trans-Blot® Turbo™ Base
1704152
Blotting instrument base, does not include any cassettes |
耗材试剂套装:
Use the Trans-Blot Turbo RTA transfer kit for fast, efficient protein transfer with the Trans-Blot Turbo rapid western blotting transfer system.
- Economical transfer option for high-throughput labs
- Optimized buffer, membrane, and filter paper combination
- PVDF membrane reduces nonspecific binding
- For 40 mini-gel transfers; requires assembly
Related Products
View our wide selection of transfer membrane sandwiches for the Trans-Blot Turbo system:
- Preassembled transfer packs for 10 transfers
- Ready-to-assemble kits for 40 transfers
- PVDF, low fluorescence (LF) PVDF, or nitrocellulose membranes
Trans-Blot Turbo
Transfer Packs and Kits |
Preassembled Transfer Packs
(10 Transfers) |
Ready-to-Assemble Transfer Kits
(40 Transfers) |
Membrane Type |
Mini |
Midi |
Mini |
Midi |
0.2 µm PVDF |
1704156 |
1704157 |
1704272 |
1704273 |
0.45 µm LF
(low fluorescence) PVDF |
— |
— |
1704274 |
1704275 |
0.2 µm Nitrocellulose |
1704158 |
1704159 |
1704270 |
1704271 |
TGX凝胶订购信息:
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Any kD™ Mini-PROTEAN® TGX™ Precast Protein Gels, 10-well, 30 µl
4569033
Pkg of 10, Any kD precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells |
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4–20% Mini-PROTEAN® TGX™ Precast Protein Gels, 10-well, 30 µl
4561093
Pkg of 10, 4–20% precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells |
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4–15% Mini-PROTEAN® TGX™ Precast Protein Gels, 10-well, 30 µl
4561083
Pkg of 10, 4–15% precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells |
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4–20% Mini-PROTEAN® TGX™ Precast Protein Gels, 15-well, 15 µl
4561096
Pkg of 10, 4–20% precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells |
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4–15% Mini-PROTEAN® TGX™ Precast Protein Gels, 15-well, 15 µl
4561086
Pkg of 10, 4–15% precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells |
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10% Mini-PROTEAN® TGX™ Precast Protein Gels, 10-well, 30 µl
4561033
Pkg of 10, 10% precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells |
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7.5% Mini-PROTEAN® TGX™ Precast Protein Gels, 10-well, 30 µl
4561023
Pkg of 10, 7.5% precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells |
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12% Mini-PROTEAN® TGX™ Precast Protein Gels, 10-well, 30 µl
4561043
Pkg of 10, 12% precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells |
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10% Mini-PROTEAN® TGX™ Precast Protein Gels, 15-well, 15 µl
4561036
Pkg of 10, 10% precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells |
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12% Mini-PROTEAN® TGX™ Precast Protein Gels, 15-well, 15 µl
4561046
Pkg of 10, 12% precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells |
蛋白电泳转印设备选型比较:
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槽 |
半干 |
iBlot |
Trans-Blot® Turbo™ 系统 |
转移速度 |
• |
•• |
••• |
•••• |
灵活性 |
• |
•• |
•• |
••• |
对于高分子量的转移效率 |
••• |
• |
•• |
••• |
通量 |
2 个小型凝胶 |
6 个小型/3 个中型凝胶 |
2 个小型/1 个中型凝胶 |
4 个小型/2 个中型凝胶 |
电源要求 |
外部电源 (HC) |
外部电源 (HC) |
集成式电源 |
集成式电源 |
用户界面 |
无 |
无 |
仅预设实验方案 |
预设实验方案和轻松定制 |
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进口|品牌 半干转印槽
编号 |
型号 |
产品名称 |
价格 |
备注说明 |
001 |
170-3940 |
美国Bio-rad伯乐Trans-Blot SD 半干转印槽 ★★ |
16360.00 |
技术参数 |
002 |
170-4150 |
美国BIO-RAD伯乐 Trans-Blot® Turbo™ 全能型蛋白转印系统★★★★ |
55000.00 |
技术参数 |
003 |
TE70XP |
美国Hoefer 半干转印电泳仪,转印面积:14×16cm,1~4块迷你胶,含电源 |
21000.00 |
技术参数 |
004 |
TE77XP |
美国Hoefer 半干转印电泳仪,转印面积:21×26cm,1~4块大胶,12块迷你胶,含电源 |
29000.00 |
技术参数 |
005 |
TE70X |
美国Hoefer 半干转印电泳仪,转印面积:14×16cm,1~4块迷你胶,无电源 |
16000.00 |
技术参数 |
006 |
TE77X |
美国Hoefer 半干转印电泳仪,转印面积:21×26cm,1~4块大胶,12块迷你胶,无电源 |
23000.00 |
技术参数 |
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国产|品牌 半干转印槽
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