仪器介绍 重新设计的 Sub-Cell GT 电泳槽提供了更新功能,可使电泳分离更加容易。 在 Sub-Cell 系列产品中,Sub-Cell GT 电泳槽是最通用的浸没式水平电泳槽。 由于 Sub-Cell GT 电泳槽可提供较好的凝胶长度、样本梳和分离模式选择,因此它非常适合 DNA 转印和 RNA 转印实验方案。 在 25 cm 距离内最多可分解 30 个样本。使用四排电泳梳,电泳槽将最多运行 120 个分析样本。
所有 Sub-Cell GT 电泳槽都包括一个缓冲液槽、一个带电缆的安全盖、一个水准气泡和多个梳(15 孔和 20 孔)。 还提供了包括其他附件的系统配置;有关更多详细信息,请参见“Sub-Cell GT 完整系统选择指南”。
功能
- QuickSnap* 电极拆卸方便,简化了清洁工作
- 底座侧面的箭头指示运行方向,可确保凝胶的方向正确
- 彩色编码的带标记的电极和带标记的底座确保盖子可以正确安置在底座上
- 通过基部上的拉环,可轻松移除盖子,从而减少缓冲液溢出量,还可防止盖位置不正确
- 反向兼容设计允许将这些电泳槽用于较早型号的组件
- 透明塑料结构,可以方便地查看样本
- 具有荧光标尺的紫外透明凝胶托盘
- 用于直接在槽中灌制您自己的凝胶的凝胶灌制口,或者用于无胶条式灌制的可选制胶盘
- 可满足一切需求的梳(与多通道吸管兼容的梳、高度固定的嵌入式梳、可调节高度的梳和制备梳)
Sub-Cell GT 完整系统
货号 |
浇注门 |
凝胶
制胶盘 |
UVTP 托盘 (cm) |
PowerPac Basic
电源 |
15 x 10 |
15 x 15* |
15 x 20 |
15 x 25 |
Sub-Cell GT 系统 |
170-4401 |
|
|
• |
|
|
|
|
170-4402 |
• |
|
|
• |
|
|
|
170-4403 |
|
|
|
|
• |
|
|
170-4404 |
|
|
|
|
|
• |
|
170-4481 |
|
• |
• |
|
|
|
|
170-4482 |
• |
• |
|
• |
|
|
|
170-4483 |
|
• |
|
|
• |
|
|
170-4484 |
|
• |
|
|
|
• |
|
164-0302 |
• |
• |
|
• |
|
|
• |
* 15 x 15 cm 样品盘允许使用浇注口在电泳槽中浇注凝胶。 可使用制胶盘灌注其他尺寸的凝胶。 |
Sub-Cell GT 电泳梳选择指南
货号 |
孔数量 |
高度 |
厚度 |
宽度孔 |
长度
牙齿 |
体积
(在 5 mm 深凝胶中) |
Sub-Cell GT 系统的梳 |
170-4449 |
30 |
固定 |
1.5 mm |
2.7 mm |
14 mm |
20.25 µl |
170-4344 |
30 |
可调 |
1.5 mm |
2.7 mm |
19.2 mm |
20.25 µl |
170-4447 |
20 |
固定 |
0.75 mm |
5 mm |
15 mm |
18.75 µl |
170-4448** |
20 |
固定 |
1.5 mm |
5 mm |
15 mm |
37.5 µl |
170-4321 |
20 |
可调 |
0.75 mm |
4.8 mm |
19.1 mm |
17 µl |
170-4322 |
15 |
可调 |
1.5 mm |
4.8 mm |
19.1 mm |
34 µl |
170-4445 |
15 |
固定 |
0.75 mm |
6 mm |
15 mm |
22.5 µl |
170-4446** |
15 |
固定 |
1.5 mm |
6 mm |
15 mm |
45 µl |
170-4323 |
15 |
可调 |
0.75 mm |
5.5 mm |
19.1 mm |
21 µl |
170-4324 |
15 |
可调 |
1.5 mm |
5.5 mm |
19.1 mm |
42 µl |
170-4443 |
10 |
固定 |
0.75 mm |
10 mm |
14 mm |
37.5 µl |
170-4444 |
10 |
固定 |
1.5 mm |
10 mm |
14 mm |
75 µl |
170-4325 |
10 |
可调 |
0.75 mm |
9.9 mm |
19.1 mm |
37 µl |
170-4326 |
10 |
可调 |
1.5 mm |
9.9 mm |
19.1 mm |
74 µl |
|
Sub-Cell GT 系统的制备梳 |
170-4442 |
4 制备 |
固定 |
1.5 mm |
27 mm |
14 mm |
202 µl |
|
2 参考 |
|
|
3 mm |
|
22 µl |
170-4441 |
2 制备 |
可调 |
1.5 mm |
50 mm |
14 mm |
375 µl |
|
2 参考 |
|
|
4 mm |
|
30 µl |
170-4440 |
1 制备 |
固定 |
1.5 mm |
106 mm |
20 mm |
795 µl |
|
2 参考 |
|
|
4 mm |
|
30 µl |
170-4328 |
1 制备 |
可调 |
3 mm |
106 mm |
19.1 mm |
1,590 µl |
|
2 参考 |
|
|
4 mm |
|
60 µl |
|
用于 Sub-Cell GT 系统的、与多通道移液器相兼容的电泳梳 |
170-4456 |
26 |
固定 |
0.75 mm |
3 mm |
15 mm |
11.25 µl |
170-4457 |
26 |
固定 |
1.5 mm |
3 mm |
15 mm |
22.5 µl |
170-4454 |
18 |
固定 |
0.75 mm |
3 mm |
14 mm |
11.25 µl |
170-4455 |
18 |
固定 |
1.5 mm |
3 mm |
14 mm |
22.5 µl |
170-4452 |
14 |
固定 |
0.75 mm |
6 mm |
20 mm |
22.5 µl |
170-4453 |
14 |
固定 |
1.5 mm |
6 mm |
20 mm |
45 µl |
170-4450 |
10 |
固定 |
0.75 mm |
6 mm |
14 mm |
22.5 µl |
170-4451 |
10 |
固定 |
1.5 mm |
6 mm |
14 mm |
45 µl |
技术参数:
电泳槽尺寸(宽 x 长 x 高) |
18 x 40.5 x 9.4 厘米 |
凝胶托盘尺寸 (OD)(宽 x 长) |
15 x 10 厘米
15 x 15 厘米
15 x 20 厘米
15 x 25 厘米 |
是否支持 ReadyAgarose™ 凝胶 |
否 |
样本通量 |
1–120 |
基本缓冲液需要量 |
~1 L |
缓冲液再循环 |
否 |
溴酚蓝迁移 |
~3.0 cm/hr(75 V 下) |
订购信息:
|
Sub-Cell GT System 1704401
Horizontal electrophoresis system, includes 15- and 20-well combs, 15 x 10 cm UV-transparent tray |
|
Sub-Cell GT System 1704402
Horizontal electrophoresis system, includes 15- and 20-well combs, 15 x 15 cm UV-transparent tray, casting gates |
|
|
|
|
|
Sub-Cell GT System 1704403
Horizontal electrophoresis system, includes 15- and 20-well combs, 15 x 20 cm UV-transparent tray |
|
Sub-Cell GT System 1704404
Horizontal electrophoresis system, includes 15- and 20-well combs, 15 x 25 cm UV-transparent tray |
|
|
|
|
|
Sub-Cell GT System 1704481
Horizontal electrophoresis system, includes 15- and 20-well combs, 15 x 10 cm UV-transparent tray, gel caster |
|
Sub-Cell® GT System 1704482
Horizontal electrophoresis system, includes 15- and 20-well combs, 15 x 15 cm UV-transparent tray, casting gates, gel caster |
|
|
|
|
|
Sub-Cell GT System 1704483
Horizontal electrophoresis system, includes 15- and 20-well combs, 15 x 20 cm UV-transparent tray, gel caster |
|
Sub-Cell® GT System 1704484
Horizontal electrophoresis system, includes 15- and 20-well combs, 15 x 25 cm UV-transparent tray, gel caster |
梳子订购:
|
30-Well Comb 1704449
Pkg of 1, 14 cm wide, 1.5 mm thick, fixed-height comb, for use with Sub-Cell and wide Mini-Sub cell GT systems, must use with GT gel tray |
 |
30-Well Comb 1704344
Pkg of 1, 14 cm wide, 1.5 mm thick, adjustable-height comb, for use with Sub-Cell and wide Mini-Sub cell GT systems, requires comb holder (#170-4320) |
|
|
|
|
|
20-Well Comb 1704447
Pkg of 1, 14 cm wide, 0.75 mm thick, fixed-height comb, for use with Sub-Cell and wide Mini-Sub cell GT systems, must use with GT gel tray |
|
20-Well Comb 1704448
Pkg of 1, 14 cm wide, 1.5 mm thick, fixed-height comb, for use with Sub-Cell and wide Mini-Sub cell GT systems, must use with GT gel tray |
|
|
|
|
|
20-Well Comb 1704321
Pkg of 1, 14 cm wide, 0.75 mm thick, adjustable-height comb, for use with Sub-Cell and wide Mini-Sub cell GT systems, requires comb holder (#170-4320) |
|
20-Well Comb 1704322
Pkg of 1, 14 cm wide, 1.5 mm thick, adjustable-height comb, for use with Sub-Cell and wide Mini-Sub cell GT systems, requires comb holder (#170-4320) |
|
|
|
|
|
15-Well Comb 1704445
Pkg of 1, 14 cm wide, 0.75 mm thick, fixed-height comb, for use with Sub-Cell and wide Mini-Sub cell GT systems, must use with GT gel tray |
|
15-Well Comb 1704446
Pkg of 1, 14 cm wide, 1.5 mm thick, fixed-height comb, for use with Sub-Cell and wide Mini-Sub cell GT systems, must use with GT gel tray |
|
|
|
|
|
15-Well Comb 1704323
Pkg of 1, 14 cm wide, 0.75 mm thick, adjustable-height comb, for use with Sub-Cell and wide Mini-Sub cell GT systems, requires comb holder (#170-4320) |
|
15-Well Comb 1704324
Pkg of 1, 14 cm wide, 1.5 mm thick, adjustable-height comb, for use with Sub-Cell and wide Mini-Sub cell GT systems, requires comb holder (#170-4320) |
|
|
|
|
|
10-Well Comb 1704443
Pkg of 1, 14 cm wide, 0.75 mm thick, fixed-height comb, for use with Sub-Cell and wide Mini-Sub cell GT systems, must use with GT gel tray |
|
10-Well Comb 1704444
Pkg of 1, 14 cm wide, 1.5 mm thick, fixed-height comb, for use with Sub-Cell and wide Mini-Sub cell GT systems, must use with GT gel tray |
|
|
|
|
|
10-Well Comb 1704325
Pkg of 1, 14 cm wide, 0.75 mm thick, adjustable-height comb, for use with Sub-Cell and wide Mini-Sub cell GT systems, requires comb holder (#170-4320) |
|
10-Well Comb 1704326
Pkg of 1, 14 cm wide, 1.5 mm thick, adjustable-height comb, for use with Sub-Cell and wide Mini-Sub cell GT systems, requires comb holder (#170-4320) |
|
|
|
|
|
Preparative Comb for Sub-Cell and Wide Mini-Sub Cell Systems 1704442
Pkg of 1, 1.5 mm thick, preparative comb with 4 preparative and 2 reference wells, fixed height, must use with GT gel tray |
|
Preparative Comb for Sub-Cell and Wide Mini-Sub Cell Systems 1704441
Pkg of 1, 1.5 mm thick, preparative comb with 2 preparative and 2 reference wells, adjustable height |
|
|
|
|
|
Preparative Comb for Sub-Cell and Wide Mini-Sub Cell Systems 1704440
Pkg of 1, 1.5 mm thick, preparative comb with 1 preparative and 2 reference wells, fixed height, must use with GT gel tray |
|
Preparative Comb for Sub-Cell and Wide Mini-Sub Cell Systems 1704328
Pkg of 1, 3 mm thick, preparative comb with 1 preparative and 2 reference wells, adjustable height, requires comb holder (#170-4320) |
|
|
|
|
|
Multichannel Pipet–Compatible Comb 1704456
Pkg of 1, 0.75 mm thick, fixed height 26-well comb, for use with Sub-Cell and wide Mini-Sub cell GT systems, must use with GT gel tray |
|
Multichannel Pipet–Compatible Comb 1704457
Pkg of 1, 1.5 mm thick, fixed height 26-well comb, for use with Sub-Cell and wide Mini-Sub cell GT systems, must use with GT gel tray |
|
|
|
|
|
Multichannel Pipet–Compatible Comb 1704454
Pkg of 1, 0.75 mm thick, fixed height 18-well comb, for use with Sub-Cell and wide Mini-Sub cell GT systems, must use with GT gel tray |
|
Multichannel Pipet–Compatible Comb 1704455
Pkg of 1, 1.5 mm thick, fixed height 18-well comb, for use with Sub-Cell and wide Mini-Sub cell GT systems, must use with GT gel tray |
|
|
|
|
|
Multichannel Pipet–Compatible Comb 1704452
Pkg of 1, 0.75 mm thick, fixed height 14-well comb, for use with Sub-Cell and wide Mini-Sub cell GT systems, must use with GT gel tray |
|
Multichannel Pipet–Compatible Comb 1704453
Pkg of 1, 1.5 mm thick, fixed height 14-well comb, for use with Sub-Cell and wide Mini-Sub cell GT systems, must use with GT gel tray |
|
|
|
|
|
Multichannel Pipet–Compatible Comb 1704450
Pkg of 1, 0.75 mm thick, fixed height 10-well comb, for use with Sub-Cell and wide Mini-Sub cell GT systems, must use with GT gel tray |
|
Multichannel Pipet–Compatible Comb 1704451
Pkg of 1, 1.5 mm thick, fixed height 10-well comb, for use with Sub-Cell and wide Mini-Sub cell GT systems, must use with GT gel tray |
配件:
|
Gel Caster 1704412
Pkg of 1, full size, horizontal electrophoresis agarose gel caster, for use with Sub-Cell GT systems |
 |
Sub-Cell GT UV-Transparent Gel Tray 1704416
Pkg of 1, 15 x 10 cm gel tray, 2 slots for fixed-height combs; can be used with wide Mini-Sub cell GT cell, compatible with DNA Sub-Cell system |
|
|
|
|
|
Sub-Cell GT UV-Transparent Gel Tray 1704417
Pkg of 1, 15 x 15 cm gel tray, allows casting gels in cells using casting gates, for use with Sub-Cell GT systems |
 |
Sub-Cell GT UV-Transparent Gel Tray 1704418
Pkg of 1, 15 x 20 cm gel tray, for use with Sub-Cell GT systems |
|
|
|
|
|
Sub-Cell GT UV-Transparent Gel Tray 1704419
Pkg of 1, 15 x 25 cm gel tray, for use with Sub-Cell GT systems |
 |
Sub-Cell GT Casting Gates 1704415
Pkg of 2, horizontal agarose gel electrophoresis casting gates, for use with Sub-Cell GT systems |
|
|
|
|
|
Comb Holder 1704320
Pkg of 1, horizontal electrophoresis comb holder, for use with Sub-Cell and wide Mini-Sub cell adjustable-height combs |
 |
Sub-Cell GT Anode (Red), QuickSnap Electrode Assembly 1704392
Pkg of 1, electrode assembly for use with Sub-Cell GT systems, 2009 model |
|
|
|
|
|
Sub-Cell GT Cathode (Black), QuickSnap Electrode Assembly 1704393
Pkg of 1, electrode assembly for use with Sub-Cell GT systems, 2009 model |
 |
Sub-Cell GT Anode (Red) Electrode Assembly 1704413
Pkg of 1, horizontal agarose gel electrophoresis electrode assembly, for use with Sub-Cell GT systems |
|
|
|
|
|
Sub-Cell GT Cathode (Black) Electrode Assembly 1704414
Pkg of 1, horizontal agarose gel electrophoresis electrode assembly, for use with Sub-Cell GT systems |
|
|
伯乐BIO-RAD Sub-Cell 水平电泳系统选择指南
|
|
|
|
|
|
|
Mini-Sub Cell GT* |
宽 Mini-Sub Cell GT** |
Sub-Cell GT |
Sub-Cell 96 型 |
Sub-Cell 192 型 |
电泳槽尺寸
(宽 x 长 x 高) |
9.2 x 25.5 x 5.6 厘米 |
17.8 x 25.5 x 6.8 厘米 |
18 x 40.5 x 9.4 厘米 |
29 x 30 x 9 厘米 |
29 x 40 x 9 厘米 |
凝胶托盘尺寸 (OD)
(宽 x 长) |
7 x 7 厘米
7 x 10 厘米 |
15 x 7 厘米
15 x 10 厘米 |
15 x 10 厘米
15 x 15 厘米
15 x 20 厘米
15 x 25 厘米 |
25 x 10 厘米
25 x 15 厘米 |
25 x 10 厘米
25 x 15 厘米
25 x 20 厘米
25 x 25 厘米 |
是否支持 ReadyAgarose 凝胶 |
是 |
是 |
否 |
否 |
否 |
样本通量 |
8–30*** |
10–60*** |
1–120† |
24–96*** |
24–192† |
基本缓冲液需要量 |
~270 ml |
~650 ml |
~1 L |
~2 L |
~3 L |
缓冲液再循环 |
否 |
否 |
否 |
是 |
是 |
溴酚蓝迁移 |
~4.5 cm/hr(75 V) |
~4.5 cm/hr(75 V) |
~3.0 cm/hr(75 V 下) |
~6.2 cm/hr(200 V 下) |
~5.2 cm/hr(200 V 下) |
* Mini ReadySub-Cell GT 是一种 Mini-Sub Cell GT,专用于运行 ReadyAgarose 预制胶,凝胶尺寸 7 x 10 cm。样品通量为 8,12 或 2 x 8。这种电泳槽不包括浇柱门、样品盘或电泳梳。
** 宽式 Mini ReadySub-Cell GT 是一种宽式 Mini-Sub Cell GT,专用于运行 ReadyAgarose 预制胶,凝胶尺寸 15 x 10 cm。样品通量为 20,32,2 x 32 或 4 x 26。这种电泳槽不包括浇注口、样品盘或电泳梳。
*** 样品通量值假设为每个凝胶 1–2 个电泳梳。
† 样品通量值假设为每个凝胶 1–4 个电泳梳。 |
琼脂糖凝胶灌制指南 各种凝胶尺寸的手灌选项
使 用 Sub-Cell 系列电泳槽手工灌制时可以使用两种附件: 凝胶制胶盘和浇注口。 凝胶制胶盘具有可适应多种不同尺寸的凝胶托盘的灵活性。 它们适用于整套 Sub-Cell 系统。 按每个槽的特定尺寸提供的浇注口为直接在 Sub-Cell 槽中进行浇注带来了方便。
凝胶制胶盘指南 |
托盘尺寸 (cm) |
小型凝胶制胶盘 |
全尺寸凝胶制胶盘 |
96 型制胶盘 |
192 型凝胶制胶盘 |
7 x 7 |
• |
• |
• |
• |
7 x 10 |
• |
• |
• |
• |
15 x 7 |
• |
• |
• |
• |
15 x 10 |
• |
• |
• |
• |
15 x 15 |
|
• |
• |
• |
15 x 20 |
|
• |
• |
• |
15 x 25 |
|
• |
• |
• |
25 x 10 |
|
|
• |
• |
25 x 15 |
|
|
• |
• |
25 x 20 |
|
|
|
• |
25 x 25 |
|
|
|
• |
灌制指南 |
电泳槽 |
托盘尺寸 (cm) |
灌制口 |
制胶盘 |
Mini-Sub cell GT |
7 x 7 |
• |
• |
7 x 10 |
|
• |
宽 Mini-Sub cell GT |
15 x 7 |
• |
• |
15 x 10 |
|
• |
Sub-Cell GT |
15 x 10 |
|
• |
15 x 15 |
• |
• |
15 x 20 |
|
• |
15 x 25 |
|
• |
Sub-Cell 96 型 |
25 x 10 |
• |
• |
25 x 15 |
|
• |
Sub-Cell 192 型 |
25 x 10 |
|
• |
25 x 15 |
• |
• |
25 x 20 |
|
• |
25 x 25 |
|
• |
|